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Issue 13, 2016
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Integration of a guided-mode resonance filter with microposts for in-cell protein detection

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Abstract

We present an integrated microfluidic system consisting of a label-free biosensor of a guided-mode resonance filter (GMRF) and a microfluidic channel with a micropost filter. The GMRF was fabricated through replica molding using an ultraviolet-curable polymer and a plastic substrate. An array of microposts (a diameter and height of 26.5 and 56 μm, respectively, and a spacing between 7.5 and 9.5 μm), fabricated on a silicon substrate through photolithography, was used as the filter. A double-sided tape was used to laminate the GMRF and a microfluidic chip such that the integrated device provides two functions: filtration of the cell debris and quantification of the in-cell protein concentration. By measuring the changes in the resonant wavelength from the GMRF, the detection of β-actin in an unprocessed lysed cell sample was demonstrated; the cell debris was separated using the micropost filter to prevent false measurement.

Graphical abstract: Integration of a guided-mode resonance filter with microposts for in-cell protein detection

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Supplementary files

Article information


Submitted
05 Jan 2016
Accepted
29 Apr 2016
First published
29 Apr 2016

Analyst, 2016,141, 4189-4195
Article type
Paper

Integration of a guided-mode resonance filter with microposts for in-cell protein detection

Y. Tu, M. Tsai, I. Lee, H. Hsu and C. Huang, Analyst, 2016, 141, 4189
DOI: 10.1039/C6AN00023A

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