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Issue 12, 2016
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Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation

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Abstract

We describe a one-step microfludics-based immunomagnetic isolation method to isolate CTCs directly from the whole blood of lung adenocarcinoma patients. This method avoids harsh sample preparation and enrichment steps, and therefore preserves the viability of CTCs during the in vitro isolation. Importantly, isolated, magnetic bead-bearing CTCs are concentrated in a small volume of culture medium with a high CTC density. High cell viability and culturing density promote the ex vivo expansion of limited numbers of CTCs. Expanded CTCs are characterized at the genetic, protein and metabolic levels.

Graphical abstract: Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation

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Article information


Submitted
12 Dec 2015
Accepted
09 Feb 2016
First published
09 Feb 2016

Analyst, 2016,141, 3621-3625
Article type
Communication

Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation

Z. Wang, W. Wu, Z. Wang, Y. Tang, Y. Deng, L. Xu, J. Tian and Q. Shi, Analyst, 2016, 141, 3621
DOI: 10.1039/C5AN02554K

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