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Issue 7, 2016
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Dual-emissive fluorescence measurements of hydroxyl radicals using a coumarin-activated silica nanohybrid probe

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Abstract

This work reports a novel dual-emissive fluorescent probe based on dye hybrid silica nanoparticles for ratiometric measurement of the hydroxyl radical (˙OH). In the probe sensing system, the blue emission of coumarin dye (coumarin-3-carboxylic acid, CCA) immobilized on the nanoparticle surface is selectively enhanced by ˙OH due to the formation of a coumarin hydroxylation product with strong fluorescence, whereas the emission of red fluorescent dye encapsulated in the silica nanoparticle is insensitive to ˙OH as a self-referencing signal, and so the probe provides a good quantitative analysis based on ratiometric fluorescence measurement with a detection limit of 1.65 μM. Moreover, the probe also shows high selectivity for ˙OH determination against metal ions, other reactive oxygen species and biological species. More importantly, it exhibits low cytotoxicity and high biocompatibility in living cells, and has been successfully used for cellular imaging of ˙OH, showing its promising application for monitoring of intracellular ˙OH signaling events.

Graphical abstract: Dual-emissive fluorescence measurements of hydroxyl radicals using a coumarin-activated silica nanohybrid probe

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Publication details

The article was received on 01 Nov 2015, accepted on 23 Feb 2016 and first published on 23 Feb 2016


Article type: Paper
DOI: 10.1039/C5AN02261D
Analyst, 2016,141, 2296-2302

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    Dual-emissive fluorescence measurements of hydroxyl radicals using a coumarin-activated silica nanohybrid probe

    S. Liu, J. Zhao, K. Zhang, L. Yang, M. Sun, H. Yu, Y. Yan, Y. Zhang, L. Wu and S. Wang, Analyst, 2016, 141, 2296
    DOI: 10.1039/C5AN02261D

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