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Issue 1, 2016
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Visual detection of serum asialohaptoglobin by plasmonic sandwich ELLSA – a new platform for cirrhosis diagnosis

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Abstract

The cirrhotic condition of the liver has long been acknowledged as the preface to liver cancer. The desialylation status of the serum acute phase protein, haptoglobin, has been introduced as a new diagnostic analyte for liver cirrhosis. The reliability of this new diagnostic molecule has been evaluated in 30 liver cirrhosis patients having a history of earlier viral hepatitis C (HCV-LC). A novel enzyme linked lectinosorbent assay has been developed coupled with the plasmon mechanism of gold nanoparticle aggregation as the colorimetric read out which can visually distinguish the cirrhotic liver patients from the normal healthy and hepatitis C controls. The assay can be useful for rapid point-of-care detection, and even an untrained person can execute it without a specialized instrument. This method employs Sambucus nigra agglutinin (SNA) to detect the extent of α-2,6 sialylation of serum haptoglobin, the new diagnostic molecule for liver cirrhosis.

Graphical abstract: Visual detection of serum asialohaptoglobin by plasmonic sandwich ELLSA – a new platform for cirrhosis diagnosis

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Publication details

The article was received on 28 Sep 2015, accepted on 30 Oct 2015 and first published on 03 Nov 2015


Article type: Communication
DOI: 10.1039/C5AN02000J
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Analyst, 2016,141, 76-84

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    Visual detection of serum asialohaptoglobin by plasmonic sandwich ELLSA – a new platform for cirrhosis diagnosis

    P. P. Bose, G. Mandal, D. Kumar, A. Duseja and B. P. Chatterjee, Analyst, 2016, 141, 76
    DOI: 10.1039/C5AN02000J

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