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Issue 2, 2016
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Naked-eye nanobiosensor for therapeutic drug monitoring of methotrexate

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Abstract

Sensing of methotrexate at clinically-relevant concentrations was achieved with a plasmon-coupling assay. In this assay, free methotrexate and folic acid Au nanoparticles competed for human dihydrofolate reductase (hDHFR)-functionalized Au nanoparticles (Au NP). The hDHFR-functionalized Au NPs were immobilized on a small glass sensor inserted in a portable 4-channel LSPR reader. This allowed rapid (minutes) and sensitive (nanomolar range) measurement of methotrexate concentration by means of total internal reflection plasmonic spectroscopy. The large bathochromic shifts of the plasmon-coupling assay led to striking colour changes visible to the naked eye for methotrexate at clinically-relevant concentrations. The results demonstrate the potential for therapeutic drug monitoring of a widely used chemotherapy agent, as assessed with the naked eye.

Graphical abstract: Naked-eye nanobiosensor for therapeutic drug monitoring of methotrexate

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Article information


Submitted
17 May 2015
Accepted
28 Jul 2015
First published
29 Jul 2015

Analyst, 2016,141, 697-703
Article type
Paper

Naked-eye nanobiosensor for therapeutic drug monitoring of methotrexate

H. Yockell-Lelièvre, N. Bukar, J. L. Toulouse, J. N. Pelletier and J.-F. Masson, Analyst, 2016, 141, 697
DOI: 10.1039/C5AN00996K

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