Jump to main content
Jump to site search

Issue 21, 2015
Previous Article Next Article

Electrochemically monitoring the antibiotic susceptibility of Pseudomonas aeruginosa biofilms

Author affiliations

Abstract

The condition of cells in Pseudomonas aeruginosa biofilms was monitored via the electrochemical detection of the electro-active virulence factor pyocyanin in a fabricated microfluidic growth chamber coupled with a disposable three electrode cell. Cells were exposed to 4, 16, and 100 mg L−1 colistin sulfate after overnight growth. At the end of testing, the measured maximum peak current (and therefore pyocyanin concentration) was reduced by approximately 68% and 82% in P. aeruginosa exposed to 16 and 100 mg L−1 colistin sulfate, respectively. Samples were removed from the microfluidic chamber, analyzed for viability using staining, and streaked onto culture plates to confirm that the P. aeruginosa cells were affected by the antibiotics. The correlation between electrical signal drop and the viability of P. aeruginosa cells after antibiotic exposure highlights the usefulness of this approach for future low cost antibiotic screening applications.

Graphical abstract: Electrochemically monitoring the antibiotic susceptibility of Pseudomonas aeruginosa biofilms

Back to tab navigation

Supplementary files

Publication details

The article was received on 06 Jul 2015, accepted on 08 Sep 2015 and first published on 23 Sep 2015


Article type: Paper
DOI: 10.1039/C5AN01358E
Author version
available:
Download author version (PDF)
Citation: Analyst, 2015,140, 7195-7201
  • Open access: Creative Commons BY license
  •   Request permissions

    Electrochemically monitoring the antibiotic susceptibility of Pseudomonas aeruginosa biofilms

    T. A. Webster, H. J. Sismaet, I. J. Chan and E. D. Goluch, Analyst, 2015, 140, 7195
    DOI: 10.1039/C5AN01358E

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements