Determination of hematocrit by voltage-induced hemolysis on a disposable electrochemical sensing strip†
Besides being a crucial parameter for surgery and clinical diagnosis, hematocrit tends to affect the analytical results of point-of-care analytical devices. Therefore, an accurate and quick method for measuring hematocrit was developed on the basis of screen-printed carbon electrodes (SPCE). An impulse DC voltage of 3.0 V was imposed on ferricyanide-coated SPCE to induce hemolysis, and the released hemoglobin reduced the ferricyanide, generating a higher oxidation current for estimating hematocrit. Hematocrit ranging from 10 to 70% can be determined in 5 s by linear sweep voltammetry (r2 = 0.9907) or 0.8 s by 3 V of potential step voltammetry (r2 = 0.9833).