Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 22nd May 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 22, 2015
Previous Article Next Article

Electrochemical immunosensor for sensitive determination of the anorexigen peptide YY at grafted reduced graphene oxide electrode platforms

Author affiliations

Abstract

The first electrochemical immunosensor for the determination of peptide YY is reported in this paper. A novel electrochemical platform, prepared by the electrochemical grafting of the diazonium salt of 4-aminobenzoic acid onto a reduced graphene oxide-modified glassy carbon electrode, was used, on which the covalent immobilization of specific anti-PYY antibodies was accomplished. The HOOC-Phe-rGO/GCEs were characterized using cyclic voltammetry and electrochemical impedance spectroscopy. The different variables affecting the preparation of the modified electrodes and the performance of the immunosensor were optimized. Under the optimized conditions, a calibration plot for PYY showing a linear range extending between 10−4 and 102 ng mL−1 was found. This range is adequate for the determination of this protein in real samples, since the expected concentration in human serum is around 100 pg mL−1. The limit of detection was 0.01 pg mL−1 of PYY. The immunosensor exhibited good reproducibility of the PYY measurements, excellent storage stability and selectivity, as well as a shorter assay time than those of ELISA kits. The usefulness of the immunosensor for the analysis of real samples was demonstrated by analyzing human serum samples spiked with PYY at three concentration levels.

Graphical abstract: Electrochemical immunosensor for sensitive determination of the anorexigen peptide YY at grafted reduced graphene oxide electrode platforms

Back to tab navigation

Supplementary files

Publication details

The article was received on 13 Jun 2015, accepted on 30 Jul 2015 and first published on 31 Jul 2015


Article type: Paper
DOI: 10.1039/C5AN01185J
Author version
available:
Download author version (PDF)
Analyst, 2015,140, 7527-7533

  •   Request permissions

    Electrochemical immunosensor for sensitive determination of the anorexigen peptide YY at grafted reduced graphene oxide electrode platforms

    S. Guerrero, G. Martínez-García, V. Serafín, L. Agüí, P. Yáñez-Sedeño and J. M. Pingarrón, Analyst, 2015, 140, 7527
    DOI: 10.1039/C5AN01185J

Search articles by author

Spotlight

Advertisements