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Issue 16, 2015
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An ultralow background substrate for protein microarray technology

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Abstract

We herein report an ultralow background substrate for protein microarrays. Conventional protein microarray substrates often suffer from non-specific protein adsorption and inhomogeneous spot morphology. Consequently, surface treatment and a suitable printing solution are required to improve the microarray performance. In the current work, we improved the situation by developing a new microarray substrate based on a fluorinated ethylene propylene (FEP) membrane. A polydopamine microspot array was fabricated on the FEP membrane, with proteins conjugated to the FEP surface through polydopamine. Uniform microspots were obtained on FEP without the application of a special printing solution. The modified FEP membrane demonstrated ultralow background signal and was applied in protein and peptide microarray analysis.

Graphical abstract: An ultralow background substrate for protein microarray technology

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Supplementary files

Article information


Submitted
30 Apr 2015
Accepted
15 Jun 2015
First published
16 Jun 2015

Analyst, 2015,140, 5627-5633
Article type
Paper
Author version available

An ultralow background substrate for protein microarray technology

H. Feng, Q. Zhang, H. Ma and B. Zheng, Analyst, 2015, 140, 5627
DOI: 10.1039/C5AN00852B

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