Jump to main content
Jump to site search

Issue 11, 2015
Previous Article Next Article

Stabilization of Prussian blue with polyaniline and carbon nanotubes in neutral media for in vivo determination of glucose in rat brains

Author affiliations

Abstract

This study demonstrates a new electrochemical microbiosensor for selective in vivo monitoring of glucose in rat brains. The microbiosensor is prepared by using Prussian blue (PB)/polyaniline (PANI)/multi-walled carbon nanotubes (MWNTs) as the electrocatalyst for the reduction and determination of H2O2 generated from the glucose oxidase (GOx)-based enzymatic catalytic reaction. PANI and MWNTs are used to stabilize PB nanoparticles in physiological solutions. As a result, the as-formed three-dimensional (3D) PB/PANI/MWNT nanostructure exhibits a stable and large electrochemical response compared to the PB-modified electrode. The use of PB/PANI/MWNTs in this work to replace “natural peroxidase” (i.e., horseradish peroxidase) used in the existing microbiosensors enables the method developed here to be facile but selective for in vivo measurements of glucose virtually interference-free from ascorbic acid and other electroactive species coexisting in the brain. This property, along with the good linearity and stability toward glucose, makes this microbiosensor competent for continuous in vivo monitoring of the changes of glucose in rat brains during intraperitoneal injection of insulin. The method demonstrated here can be applied to develop other oxidase-based microbiosensors for other neurochemicals, which would be helpful for understanding the chemical process involved in some physiological and pathological events.

Graphical abstract: Stabilization of Prussian blue with polyaniline and carbon nanotubes in neutral media for in vivo determination of glucose in rat brains

Back to tab navigation

Publication details

The article was received on 21 Dec 2014, accepted on 16 Jan 2015 and first published on 19 Jan 2015


Article type: Paper
DOI: 10.1039/C4AN02352H
Citation: Analyst, 2015,140, 3746-3752
  •   Request permissions

    Stabilization of Prussian blue with polyaniline and carbon nanotubes in neutral media for in vivo determination of glucose in rat brains

    R. Li, D. Guo, J. Ye and M. Zhang, Analyst, 2015, 140, 3746
    DOI: 10.1039/C4AN02352H

Search articles by author

Spotlight

Advertisements