Issue 8, 2015

Versatile G-quadruplex-mediated strategies in label-free biosensors and logic systems

Abstract

G-quadruplex (G4), as one of the significant functional nucleic acids (FNAs), has attracted researchers’ wide attention, and in particular has been employed for the construction of label-free molecular sensors and logic systems based on the peroxidase-like activity of the G4–hemin complex and G4-enhanced luminescence of G4-binding organic dyes. Its cation-dependent conformation and stability provide opportunities for the recognition of metal ion inputs and application of a split G4 strategy. Moreover, coupling the G4 sequence with other FNAs, e.g. metal ion-dependent DNAzymes and aptamers, has prominently broadened the range of possible targets from metal ions and DNA to diverse proteins and cells. Although there are limitations, such as a low ability of anti-interference and multiplex analysis, the excellent advantages (e.g. simplicity and low cost) endow the G4-mediated strategy with tremendous potential to be further exploited for practical bioanalysis and complicated DNA computing.

Graphical abstract: Versatile G-quadruplex-mediated strategies in label-free biosensors and logic systems

Article information

Article type
Critical Review
Submitted
12 Dec 2014
Accepted
02 Feb 2015
First published
03 Feb 2015

Analyst, 2015,140, 2556-2572

Versatile G-quadruplex-mediated strategies in label-free biosensors and logic systems

J. Ren, T. Wang, E. Wang and J. Wang, Analyst, 2015, 140, 2556 DOI: 10.1039/C4AN02282C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements