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Issue 7, 2015
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Comparison of transmission and transflectance mode FTIR imaging of biological tissue

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Abstract

FTIR microscopy is a powerful technique which has become popular due to its ability to provide complementary information during histopathological assessment of biomedical tissue samples. Recently however, questions have been raised on the suitability of the transflection mode of operation for clinical diagnosis due to the so called Electric Field Standing Wave (EFSW) effect. In this paper we compare chemical images measured in transmission and transflection from prostate tissue obtained from five different patients, and discuss the variability of the spectra acquired with each sampling modality. We find that spectra obtained in transflection undergo a non-linear distortion, i.e. non-linear variations in absorption band strength across the spectra, and that there are significant differences in spectra measured from the same area of tissue depending on the mode of operation. Principal Component Analysis (PCA) is used to highlight that poorer discrimination between benign and cancerous tissue is obtained in transflection mode. In addition we show that use of second derivatives, while qualitatively improves spectral discrimination, does not completely alleviate the underlying problem.

Graphical abstract: Comparison of transmission and transflectance mode FTIR imaging of biological tissue

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Article information


Submitted
29 Oct 2014
Accepted
02 Feb 2015
First published
02 Feb 2015

Analyst, 2015,140, 2383-2392
Article type
Paper
Author version available

Comparison of transmission and transflectance mode FTIR imaging of biological tissue

M. J. Pilling, P. Bassan and P. Gardner, Analyst, 2015, 140, 2383
DOI: 10.1039/C4AN01975J

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