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Issue 7, 2015
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Analysis of the developing neural system using an in vitro model by Raman spectroscopy

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Abstract

We developed an in vitro model of early neural cell development. The maturation of a normal neural cell was studied in vitro using Raman spectroscopy for 120 days. The Raman spectra datasets were analyzed by principal component analysis (PCA) to investigate the relationship between maturation stages and molecular composition changes in neural cells. According to the PCA, the Raman spectra datasets can be classified into four larger groups. Previous electrophysiological studies have suggested that a normal neural cell goes through three maturation states. The groups we observed by Raman analysis showed good agreement with the electrophysiological results, except with the addition of a fourth state. The results demonstrated that Raman analysis was powerful to investigate the daily changes in molecular composition of the growing neural cell. This in vitro model system may be useful for future studies of the effects of endocrine disrupters in the developing early neural system.

Graphical abstract: Analysis of the developing neural system using an in vitro model by Raman spectroscopy

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Article information


Submitted
27 Oct 2014
Accepted
14 Jan 2015
First published
14 Jan 2015

Analyst, 2015,140, 2344-2349
Article type
Paper
Author version available

Analysis of the developing neural system using an in vitro model by Raman spectroscopy

K. Hashimoto, S. N. Kudoh and H. Sato, Analyst, 2015, 140, 2344
DOI: 10.1039/C4AN01961J

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