Issue 6, 2015
From the journal:

Analyst

An amplified fluorescence detection of T4 polynucleotide kinase activity based on coupled exonuclease III reaction and a graphene oxide platform†

Ni-Na Sun,a   Rong-Mei Kong,*ab   Fengli Qu,a   Xiaobing Zhang,b   Shufang Zhanga  and  Jinmao You*a  

* Corresponding authors

a The Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu Shandong 273165, P. R. China
E-mail: kongrongmei@126.com, jmyou6304@163.com
Fax: +86-537-4456301
Tel: +86-537-4456301

b State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China

Abstract

A novel amplified fluorescence graphene oxide (GO) sensing platform for sensitive detection of T4 polynucleotide kinase (PNK) activity and inhibition was developed based on the exonuclease III (ExoIII) reaction. The efficient digestion capacity of ExoIII and the super quenching ability of GO both contribute to the detection sensitivity.

Graphical abstract: An amplified fluorescence detection of T4 polynucleotide kinase activity based on coupled exonuclease III reaction and a graphene oxide platform

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Article information

DOI
https://doi.org/10.1039/C4AN01953A
Article type
Communication
Submitted
26 Oct 2014
Accepted
31 Jan 2015
First published
02 Feb 2015

Analyst, 2015,140, 1827-1831

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An amplified fluorescence detection of T4 polynucleotide kinase activity based on coupled exonuclease III reaction and a graphene oxide platform

N. Sun, R. Kong, F. Qu, X. Zhang, S. Zhang and J. You, Analyst, 2015, 140, 1827 DOI: 10.1039/C4AN01953A

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