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Issue 7, 2015
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Biochemical changes of the endothelium in the murine model of NO-deficient hypertension

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Abstract

The main spectral differences between the biochemical compositions of the vascular endothelium of control, hypertensive NO-deficient, and NO-deficient mice supplemented with nitrate were studied using Raman microimaging. A significantly different Raman signature of the endothelium in these three groups in the 1200–1400 cm−1 region was assigned to the α-helix and β-sheet alterations in the protein secondary structure upon the development of hypertension. The second pronounced biochemical marker of endothelium alterations was the lipid to protein ratio. A lower intensity of the band at 2940 cm−1 relative to the feature at 1007 cm−1 in the endothelium in hypertension compared to the control indicated a decrease of the lipid content relative to proteins during the progress of the pathology. The nitrate-based treatment partially reversed the effects of hypertension. The nitrate supplementation restored the lipid to protein ratio in the endothelium to the control level, while the changes in the secondary structure of proteins were irreversible upon nitrate administration.

Graphical abstract: Biochemical changes of the endothelium in the murine model of NO-deficient hypertension

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Article information


Submitted
17 Oct 2014
Accepted
01 Dec 2014
First published
02 Dec 2014

Analyst, 2015,140, 2178-2184
Article type
Paper
Author version available

Biochemical changes of the endothelium in the murine model of NO-deficient hypertension

M. Z. Pacia, L. Mateuszuk, S. Chlopicki, M. Baranska and A. Kaczor, Analyst, 2015, 140, 2178
DOI: 10.1039/C4AN01870B

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