Issue 22, 2014
From the journal:

Analyst

DNA-hosted Hoechst dyes: application for label-free fluorescent monitoring of endonuclease activity and inhibition†

Xiao-Qin Jiang,a   Su-Miao Guo,a   Min Zhang,*b   Ming Zhouc  and  Bang-Ce Ye*a  

* Corresponding authors

a Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, China
E-mail: bcye@ecust.edu.cn
Fax: +86-21-64252094
Tel: +86-21-64252094

b Department of Chemistry, East China Normal University, 500 Dongchuan Road, Shanghai, China
E-mail: mzhang@chem.ecnu.edu.cn
Fax: +86-21-54340042
Tel: +86-21-54340042

c Department of Chemistry, University of Washington, Seattle, WA 98195, USA

Abstract

A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.

Graphical abstract: DNA-hosted Hoechst dyes: application for label-free fluorescent monitoring of endonuclease activity and inhibition

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Article information

DOI
https://doi.org/10.1039/C4AN01373E
Article type
Communication
Submitted
25 Jul 2014
Accepted
13 Sep 2014
First published
15 Sep 2014

Analyst, 2014,139, 5682-5685

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DNA-hosted Hoechst dyes: application for label-free fluorescent monitoring of endonuclease activity and inhibition

X. Jiang, S. Guo, M. Zhang, M. Zhou and B. Ye, Analyst, 2014, 139, 5682 DOI: 10.1039/C4AN01373E

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