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Issue 21, 2014
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Haptens, bioconjugates, and antibodies for penthiopyrad immunosensing

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Abstract

Haptens, bioconjugates, and antibodies for highly sensitive immunochemical analysis of the new-generation fungicide penthiopyrad are described. Two haptens with equivalent carboxylated linkers were prepared, and the purified active esters were efficiently coupled to proteins. The results revealed slightly different antibody-eliciting capacities for the two synthetic derivatives. All of the produced antibodies were specific for penthiopyrad, and showed affinity values in the nanomolar range.

Graphical abstract: Haptens, bioconjugates, and antibodies for penthiopyrad immunosensing

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Supplementary files

Article information


Submitted
07 May 2014
Accepted
25 Aug 2014
First published
26 Aug 2014

This article is Open Access

Analyst, 2014,139, 5358-5361
Article type
Communication
Author version available

Haptens, bioconjugates, and antibodies for penthiopyrad immunosensing

E. Ceballos-Alcantarilla, A. Abad-Fuentes, V. Aloisio, C. Agulló, A. Abad-Somovilla and J. V. Mercader, Analyst, 2014, 139, 5358
DOI: 10.1039/C4AN00828F

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