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Issue 17, 2014
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Separation and sensitive determination of sphingolipids at low femtomole level by using HPLC-PIESI-MS/MS

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Abstract

A highly sensitive paired ion electrospray ionization mass spectrometry (PIESI-MS) approach was developed for the trace determination of sphingolipids. Apart from their structure role, specific sphingolipids can play a role in cell signaling and as disease markers. With the optimal pairing reagents, detection limits ranged from low femtomole to picomole levels for 14 selected sphingolipids. This improved the detection sensitivity of ESI-MS for many of these analytes up to ∼4000 times.

Graphical abstract: Separation and sensitive determination of sphingolipids at low femtomole level by using HPLC-PIESI-MS/MS

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Publication details

The article was received on 30 Apr 2014, accepted on 26 Jun 2014 and first published on 08 Jul 2014


Article type: Communication
DOI: 10.1039/C4AN00775A
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Citation: Analyst, 2014,139, 4169-4175

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    Separation and sensitive determination of sphingolipids at low femtomole level by using HPLC-PIESI-MS/MS

    C. Xu, E. C. Pinto and D. W. Armstrong, Analyst, 2014, 139, 4169
    DOI: 10.1039/C4AN00775A

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