Issue 20, 2014

Construction of carbon nanotube based nanoarchitectures for selective impedimetric detection of cancer cells in whole blood

Abstract

A carbon nanotube (CNT) based nanoarchitecture is developed for rapid, sensitive and specific detection of cancer cells by using real time electrical impedance sensing. The sensor is constructed with carbon nanotube (CNT) multilayers and EpCAM (epithelial cell adhesion molecule) antibodies, which are assembled on an indium tin oxide (ITO) electrode surface. The binding of tumor cells to EpCAM antibodies causes increase of the electron-transfer resistance. The electrochemical impedance of the prepared biosensors is linear with the logarithm of concentration of the liver cancer cell line (HepG2) within the concentration range of 10 to 105 cells per mL. The detection limit for HepG2 cells is 5 cells per mL. The proposed impedimetric sensing devices allow for sensitive and specific detection of cancer cells in whole-blood samples without any sample pretreatment steps.

Graphical abstract: Construction of carbon nanotube based nanoarchitectures for selective impedimetric detection of cancer cells in whole blood

Supplementary files

Article information

Article type
Paper
Submitted
29 Apr 2014
Accepted
21 Jul 2014
First published
21 Jul 2014

Analyst, 2014,139, 5086-5092

Construction of carbon nanotube based nanoarchitectures for selective impedimetric detection of cancer cells in whole blood

Y. Liu, F. Zhu, W. Dan, Y. Fu and S. Liu, Analyst, 2014, 139, 5086 DOI: 10.1039/C4AN00758A

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