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Issue 17, 2014
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Up-regulating pyocyanin production by amino acid addition for early electrochemical identification of Pseudomonas aeruginosa

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Abstract

This work focuses on developing a faster method for electrochemically detecting a Pseudomonas aeruginosa infection through the addition of amino acids to cell culture samples. We performed square-wave voltammetry measurements of pyocyanin produced by P. aeruginosa using commercially available carbon-based electrodes connected to a Ag/AgCl reference. The electrochemical response resulting from the production of pyocyanin by bacteria was measured in the presence of various amino acids while varying three different culturing parameters: liquid media type (trypticase soy broth vs. M63 minimal media); concentration of amino acids in the solution; and initial concentration of the P. aeruginosa in the solution. Our results demonstrate a faster and stronger electrochemical response in media containing tyrosine and valine at elevated concentrations, lending promise to using amino acids as up-regulatory molecules for faster bacterial detection.

Graphical abstract: Up-regulating pyocyanin production by amino acid addition for early electrochemical identification of Pseudomonas aeruginosa

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Supplementary files

Article information


Submitted
28 Apr 2014
Accepted
23 Jun 2014
First published
23 Jun 2014

Analyst, 2014,139, 4241-4246
Article type
Paper
Author version available

Up-regulating pyocyanin production by amino acid addition for early electrochemical identification of Pseudomonas aeruginosa

H. J. Sismaet, T. A. Webster and E. D. Goluch, Analyst, 2014, 139, 4241
DOI: 10.1039/C4AN00756E

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