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Issue 19, 2014
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Light sheet fluorescence microscopy (LSFM): past, present and future

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Abstract

Light sheet fluorescence microscopy (LSFM) has emerged as an important imaging modality to follow biology in live 3D samples over time with reduced phototoxicity and photobleaching. In particular, LSFM has been instrumental in revealing the detail of early embryonic development of Zebrafish, Drosophila, and C. elegans. Open access projects, DIY-SPIM, OpenSPIM, and OpenSPIN, now allow LSFM to be set-up easily and at low cost. The aim of this paper is to facilitate the set-up and use of LSFM by reviewing and comparing open access projects, image processing tools and future challenges.

Graphical abstract: Light sheet fluorescence microscopy (LSFM): past, present and future

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Publication details

The article was received on 07 Apr 2014, accepted on 14 Jul 2014 and first published on 21 Jul 2014


Article type: Tutorial Review
DOI: 10.1039/C4AN00624K
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Citation: Analyst, 2014,139, 4758-4768

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    Light sheet fluorescence microscopy (LSFM): past, present and future

    J. Lim, H. K. Lee, W. Yu and S. Ahmed, Analyst, 2014, 139, 4758
    DOI: 10.1039/C4AN00624K

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