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Issue 14, 2014
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An efficient nanomaterial-based electrochemical biosensor for sensitive recognition of drug-resistant leukemia cells

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Abstract

A novel electrochemical cytosensor was developed for the fast and high-sensitivity recognition of drug-resistant leukemia K562/ADM cells based on the P-glycoprotein (P-gp) expression level on a cell membrane. The nanocomposite interface of the gold nanoparticles/polyaniline nanofibers (AuNPs/PANI-NF) was chosen to design the biosensor for electrochemical detection. Au/PANI-NF-based cytosensors coated with anti-P-glycoprotein (anti-P-gp) molecules could provide a biomimetic interface for the immunosensing of cell surface P-glycoprotein, and thus could capture the over-expression P-gp cells. Transmission electron microscopy (TEM) indicated that the gold nanoparticles were uniformly anchored along the structure of the PANI-NF surface, displaying fibrillar morphology with a diameter of ∼70 nm, and atomic force microscopy (AFM) further presented the morphology of the nanocomposite film. Owing to the high affinity of anti-P-gp for leukemia K562/ADM cells of the propounded sensing platform, the proposed biosensor exhibited excellent analytical performance for leukemia K562/ADM cells, ranging from 1.6 × 102 to 1.6 × 106 cells per mL with a detection limit of 80 cells per mL. Recovery experiments indicated that the sensitivity reported here is suitable for practical application. The cell surface P-gp expression level was analysed by flow cytometric experiments, which confirmed the above recognized result. This strategy is also a cost-effective and convenient operation, implying great promise for the sensitive recognition of cancer cells and cell surface receptors; thus, it is helpful in cancer diagnosis.

Graphical abstract: An efficient nanomaterial-based electrochemical biosensor for sensitive recognition of drug-resistant leukemia cells

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Article information


Submitted
04 Mar 2014
Accepted
06 May 2014
First published
03 Jun 2014

Analyst, 2014,139, 3629-3635
Article type
Paper

An efficient nanomaterial-based electrochemical biosensor for sensitive recognition of drug-resistant leukemia cells

S. Zhang, L. Zhang, X. Zhang, P. Yang and J. Cai, Analyst, 2014, 139, 3629
DOI: 10.1039/C4AN00420E

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