Issue 14, 2014
From the journal:

Analyst

Digital colour tone for fluorescence sensing: a direct comparison of intensity, ratiometric and hue based quantification

Aron Hakonen,*a   Jonathon E. Bevesb  and  Niklas Strömbergc  

* Corresponding authors

a Department of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden
E-mail: hakonen@chalmers.se
Tel: +46 704964675

b School of Chemistry, UNSW Australia, Sydney, Australia
E-mail: j.beves@ unsw.edu.au

c SP Technical Research Institute of Sweden, Chemistry, Materials and Surfaces, Box 857, SE-501 15 Borås, Sweden
E-mail: niklas.stromberg@sp.se
Tel: +46 (0)10 516 51 68

Abstract

The hue parameter of HSV colour-space for digital imaging is shown to be accessible for convenient quantitative fluorescence imaging. A commercially available pH probe was utilized in solution and incorporated into optical μ-sensors for microscopy applications.

Graphical abstract: Digital colour tone for fluorescence sensing: a direct comparison of intensity, ratiometric and hue based quantification

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Article information

DOI
https://doi.org/10.1039/C4AN00063C
Article type
Communication
Submitted
10 Jan 2014
Accepted
31 Jan 2014
First published
31 Jan 2014

Analyst, 2014,139, 3524-3527

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Digital colour tone for fluorescence sensing: a direct comparison of intensity, ratiometric and hue based quantification

A. Hakonen, J. E. Beves and N. Strömberg, Analyst, 2014, 139, 3524 DOI: 10.1039/C4AN00063C

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