Issue 11, 2014
From the journal:

Analyst

A signal-on fluorescent assay for DNA methyltransferase activity using a methylation-resistant endonuclease†

Quang Huy Quachab  and  Bong Hyun Chung*ab  

* Corresponding authors

a BioNano Heath Guard Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O Box 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, South Korea
E-mail: chungbh@kribb.re.kr
Fax: +82-42-879-8594
Tel: +82-42-860-4442

b Nanobiotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 305-333, South Korea

Abstract

A simple, rapid, and signal-on fluorescent assay was developed for activity analysis of DNA methyltransferase and for screening of its inhibitors based on a methylation-resistant endonuclease and SYBR Green I.

Graphical abstract: A signal-on fluorescent assay for DNA methyltransferase activity using a methylation-resistant endonuclease

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Article information

DOI
https://doi.org/10.1039/C3AN02129G
Article type
Communication
Submitted
15 Nov 2013
Accepted
11 Mar 2014
First published
11 Mar 2014

Analyst, 2014,139, 2674-2677

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A signal-on fluorescent assay for DNA methyltransferase activity using a methylation-resistant endonuclease

Q. H. Quach and B. H. Chung, Analyst, 2014, 139, 2674 DOI: 10.1039/C3AN02129G

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