Issue 7, 2014

Implementation of laser induced fluorescence in a pulse radiolysis experiment – a new way to analyze resazurin-like reduction mechanisms

Abstract

Resazurin (RNO) reduction by hydrated electrons produces a fluorescent molecule: resorufin (RN). To take advantage of RN fluorescence, a novel setup is designed by implementing fluorescence detection induced by laser in a pulse radiolysis experiment. Time resolved fluorescence spectra were recorded with a fast gated intensified CCD camera during the reduction of RNO from μs to ms. Two 532 nm laser types have been used to describe the short μs range by a 5 ns Q-switch laser and the μs–ms range by a CW DPSS laser. By fitting the simulated model to the experimental data a second order rate constant of 109 M−1 s−1 was re-evaluated. This method should be considered in the near future in many in situ and real time measurements for evaluating radical production.

Graphical abstract: Implementation of laser induced fluorescence in a pulse radiolysis experiment – a new way to analyze resazurin-like reduction mechanisms

Article information

Article type
Paper
Submitted
23 Oct 2013
Accepted
10 Jan 2014
First published
13 Jan 2014

Analyst, 2014,139, 1707-1712

Implementation of laser induced fluorescence in a pulse radiolysis experiment – a new way to analyze resazurin-like reduction mechanisms

A. Balcerzyk and G. Baldacchino, Analyst, 2014, 139, 1707 DOI: 10.1039/C3AN02000B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements