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Issue 2, 2014
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Profiling of carbohydrate mixtures at unprecedented resolution using high-precision 1H-13C chemical shift measurements and a reference library

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Abstract

Complex mixtures of carbohydrates pose distinct challenges in routine and high-throughput analysis, so that only a few carbohydrate components are routinely resolved and identified in biofluids, extracts, foods and other complex mixtures. Here, we conduct precise measurements of 1H and 13C anomeric chemical shifts to construct a reference library of specific carbohydrate signals with high-resolution two-dimensional 1H-13C NMR spectra. High-resolution multidimensional NMR spectra largely abolish resolution problems in carbohydrate analysis with state-of-the-art instrumentation. Accurate measurements of anomeric 1H-13C chemical shifts at parts per billion precisions permit robust carbohydrate identification using a very limited number of instrument-independent reference values.

Graphical abstract: Profiling of carbohydrate mixtures at unprecedented resolution using high-precision 1H-13C chemical shift measurements and a reference library

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Publication details

The article was received on 10 Oct 2013, accepted on 11 Nov 2013 and first published on 11 Nov 2013


Article type: Paper
DOI: 10.1039/C3AN01922E
Analyst, 2014,139, 401-406

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    Profiling of carbohydrate mixtures at unprecedented resolution using high-precision 1H-13C chemical shift measurements and a reference library

    B. O. Petersen, O. Hindsgaul and S. Meier, Analyst, 2014, 139, 401
    DOI: 10.1039/C3AN01922E

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