Issue 17, 2013

Real time monitoring of population dynamics in concurrent bacterial growth using SIFT-MS quantification of volatile metabolites

Abstract

Population dynamics of three different bacterial species, Serratia rubidaea (R), Serratia marcescens (F) and Escherichia coli (Ec), growing in single or mixed populations in liquid media, was monitored by real time headspace quantification of volatile compounds using selected ion flow tube mass spectrometry, SIFT-MS. The three bacterial species interact with each other in a competitive fashion in a way similar to the game “rock-paper-scissors” (R-Ec-F). The concentrations of volatile metabolites (ammonia, ethanol, acetaldehyde, propanol, acetoin, acetone and acetic acid) were measured in the headspace of the individual species and of their mixtures continuously for 24 hour periods. The results demonstrate that dynamics in bacterial cultures can be monitored using SIFT-MS in real time.

Graphical abstract: Real time monitoring of population dynamics in concurrent bacterial growth using SIFT-MS quantification of volatile metabolites

Article information

Article type
Paper
Submitted
08 Mar 2013
Accepted
24 May 2013
First published
28 May 2013

Analyst, 2013,138, 4795-4801

Real time monitoring of population dynamics in concurrent bacterial growth using SIFT-MS quantification of volatile metabolites

K. Sovová, J. Čepl, A. Markoš and P. Španěl, Analyst, 2013, 138, 4795 DOI: 10.1039/C3AN00472D

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