Issue 11, 2013
From the journal:

Analyst

Nanotips for single-step preparation of DNA for qPCR analysis†

Dinesh Kalyanasundaram,a   Jong-Hoon Kim,a   Gareth Fotouhi,a   Hyun-Boo Lee,a   Morgan Hiraiwa,a   Kieseok Oh,b   Kyong-Hoon Leeb  and  Jae-Hyun Chung*a  

* Corresponding authors

a Department of Mechanical Engineering, University of Washington, Seattle, WA, USA
E-mail: jae71@uw.edu
Fax: +1-206-685-8047
Tel: +1-206-543-4355

b NanoFacture, Inc., P.O. Box 52651, Bellevue, WA, USA

Abstract

A single-step concentration and elution method is developed for detection of DNA in buffer, saliva, and blood. A nanotip capturing DNA using an electric field and capillary action is directly dissolved in buffer for qPCR analysis. The concentration yield and the relative parameters are compared with those of a commercial kit.

Graphical abstract: Nanotips for single-step preparation of DNA for qPCR analysis

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Article information

DOI
https://doi.org/10.1039/C3AN00170A
Article type
Communication
Submitted
23 Jan 2013
Accepted
03 Apr 2013
First published
04 Apr 2013

Analyst, 2013,138, 3135-3138

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Nanotips for single-step preparation of DNA for qPCR analysis

D. Kalyanasundaram, J. Kim, G. Fotouhi, H. Lee, M. Hiraiwa, K. Oh, K. Lee and J. Chung, Analyst, 2013, 138, 3135 DOI: 10.1039/C3AN00170A

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