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Issue 11, 2013
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Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

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Abstract

This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1–2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-based signal amplification of ELISA and the concentration of enzyme reaction products by cIEF.

Graphical abstract: Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

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Article information


Submitted
12 Jan 2013
Accepted
01 Apr 2013
First published
03 Apr 2013

Analyst, 2013,138, 3139-3141
Article type
Communication

Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF)

T. G. Henares, Y. Uenoyama, Y. Nogawa, K. Ikegami, D. Citterio, K. Suzuki, S. Funano, K. Sueyoshi, T. Endo and H. Hisamoto, Analyst, 2013, 138, 3139
DOI: 10.1039/C3AN00098B

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