Jump to main content
Jump to site search

Issue 3, 2013
Previous Article Next Article

A novel signal-amplified strategy based on assembly reactivation for highly specific and sensitive detection of chair-like antiparallel G-quadruplex

Author affiliations

Abstract

Specific and sensitive identification of special G-quadruplex structures is an important issue and attracts increasing interest. In this paper, a novel, signal-amplified strategy is proposed for the specific detection of G-quadruplexes, which is very different from those currently-dominating methods based on single-molecular fluorescent probes. In this strategy, a ‘special designed’ cyanine dye loses the ability of self-assembly in solution but still keeps its assembly potential. When the G-quadruplex with a specific structure is present, the assembly potential of the dye is reactivated so that it can assemble to J-aggregates. Other DNA motifs without this specific structure cannot activate its assembly potential no matter if they are double-stranded or quadruplex DNA. Since the assembly is quite specific to structure, the induced procedure of the aggregates provides high specificity for this strategy. In addition, the attached aggregates exponentially amplify the signal due to the signal stacking of those monomers within the aggregates, which then significantly enhances the sensitivity of this strategy. As a result, this strategy exhibits a highly specific and sensitive detection ability for specific G-quadruplex structures both between quadruplexes and non-quadruplexes and among diverse quadruplex motifs.

Graphical abstract: A novel signal-amplified strategy based on assembly reactivation for highly specific and sensitive detection of chair-like antiparallel G-quadruplex

Back to tab navigation

Supplementary files

Publication details

The article was received on 25 Oct 2012, accepted on 20 Nov 2012 and first published on 21 Nov 2012


Article type: Paper
DOI: 10.1039/C2AN36557J
Analyst, 2013,138, 798-804

  •   Request permissions

    A novel signal-amplified strategy based on assembly reactivation for highly specific and sensitive detection of chair-like antiparallel G-quadruplex

    W. Gai, Q. Yang, J. Xiang, W. Jiang, Q. Li, H. Sun, L. Yu, Q. Shang, A. Guan, H. Zhang and Y. Tang, Analyst, 2013, 138, 798
    DOI: 10.1039/C2AN36557J

Search articles by author

Spotlight

Advertisements