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Issue 3, 2013
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Enzyme-free colorimetric bioassay based on gold nanoparticle-catalyzed dye decolorization

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Abstract

A novel, enzyme-free and aptamer-based colorimetric platform for protein detection has been developed, which takes advantage of aptamer-functionalized magnetic beads (MBs) for target capture, concentration and separation, and aptamer-conjugated gold nanoparticle (AuNP)-catalyzed color bleaching reaction of methyl orange (MO) to generate the colorimetric signals. It was demonstrated that the proposed colorimetric sensing strategy enables simple, cost-effective, sensitive and specific thrombin detection without the use of any enhancing solutions and enzymes. Herein, by naked eye observation, we can detect the human thrombin with a detection limit of approximately 320 pM, which can be further decreased to 30 pM with the help of a UV-vis instrument. In addition, this method also works for targets with two or more binding sites.

Graphical abstract: Enzyme-free colorimetric bioassay based on gold nanoparticle-catalyzed dye decolorization

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Publication details

The article was received on 24 Sep 2012, accepted on 23 Nov 2012 and first published on 23 Nov 2012


Article type: Communication
DOI: 10.1039/C2AN36374G
Analyst, 2013,138, 760-766

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    Enzyme-free colorimetric bioassay based on gold nanoparticle-catalyzed dye decolorization

    W. Li, J. Li, W. Qiang, J. Xu and D. Xu, Analyst, 2013, 138, 760
    DOI: 10.1039/C2AN36374G

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