Issue 20, 2012
From the journal:

Analyst

Solid probe assisted nanoelectrospray ionization mass spectrometry for biological tissue diagnostics†

Mridul Kanti Mandal,*a   Kentaro Yoshimura,b   Subhrakanti Saha,a   Satoshi Ninomiya,c   Md. Obaidur Rahman,a   Zhan Yu,d   Lee Chuin Chen,c   Yasuo Shida,a   Sen Takeda,b   Hiroshi Nonamie  and  Kenzo Hiraoka*a  

* Corresponding authors

a Clean Energy Research Center, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511 Japan
E-mail: mandal@yamanashi.ac.jp, hiraoka@yamanashi.ac.jp
Tel: +81-55-220-8509

b Department of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan

c Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511 Japan

d College of Chemistry and Biology, Shenyang Normal University, Shenyang, China

e Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, Matsuyama, Japan

Abstract

To perform remote and direct sampling for mass spectrometry, solid probe assisted nanoelectrospray ionization (SPA-nanoESI) has been newly developed. After capturing the sample on the tip of the needle by sticking it to the biological tissue, the needle was inserted into the solvent-preloaded nanoESI capillary from the backside. NanoESI gave abundant ion signals for human kidney tissues and the liver of a living mouse. The method is easy to operate and versatile because any biological specimen could be sampled away from the mass spectrometer. Minimal invasiveness is another merit of this method.

Graphical abstract: Solid probe assisted nanoelectrospray ionization mass spectrometry for biological tissue diagnostics

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C2AN36006C
Article type
Communication
Submitted
24 Jul 2012
Accepted
15 Aug 2012
First published
16 Aug 2012

Analyst, 2012,137, 4658-4661

Permissions

Request permissions

Solid probe assisted nanoelectrospray ionization mass spectrometry for biological tissue diagnostics

M. K. Mandal, K. Yoshimura, S. Saha, S. Ninomiya, Md. O. Rahman, Z. Yu, L. C. Chen, Y. Shida, S. Takeda, H. Nonami and K. Hiraoka, Analyst, 2012, 137, 4658 DOI: 10.1039/C2AN36006C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements