Jump to main content
Jump to site search

Issue 23, 2012
Previous Article Next Article

Affinity surface-assisted laser desorption/ionization mass spectrometry for peptide enrichment

Author affiliations

Abstract

In this paper, we report on the functionalization of silicon nanostructured (NanoSi) surface with an organic layer of nitrilotriacetic acid (NTA) and its subsequent use as an affinity surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) interface for histidine-tagged peptide enrichment and mass spectrometry analysis. The NTA terminal groups are immobilized onto the NanoSi surface via very stable Si–C covalent bonds. The NTA-modified NanoSi (NTA-NanoSi) interface was characterized by contact angle measurements, Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The NTA-NanoSi interface has shown a good selectivity toward His-tagged peptide and permits its enrichment from an artificial mixture of both tagged and untagged peptides and its subsequent mass spectrometry detection with good signal/noise ratio.

Graphical abstract: Affinity surface-assisted laser desorption/ionization mass spectrometry for peptide enrichment

Back to tab navigation

Supplementary files

Publication details

The article was received on 15 Jun 2012, accepted on 22 Sep 2012 and first published on 25 Sep 2012


Article type: Paper
DOI: 10.1039/C2AN35803D
Analyst, 2012,137, 5527-5532

  •   Request permissions

    Affinity surface-assisted laser desorption/ionization mass spectrometry for peptide enrichment

    Y. Coffinier, N. Nguyen, H. Drobecq, O. Melnyk, V. Thomy and R. Boukherroub, Analyst, 2012, 137, 5527
    DOI: 10.1039/C2AN35803D

Search articles by author

Spotlight

Advertisements