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Issue 16, 2012
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Nanoparticle-based substrates for surface-enhanced Raman scattering detection of bacterial spores

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Abstract

The development of ultrasensitive and rapid methods for the detection of bacterial spores is important for medical diagnostics of infectious diseases. While Surface-Enhanced Raman Spectroscopic (SERS) techniques have been increasingly demonstrated for achieving this goal, a key challenge is the development of sensitive and stable SERS substrates or probes. This Minireview highlights recent progress in exploring metal nanoparticle-based substrates, especially gold nanoparticle-based substrates, for the detection of biomarkers released from bacterial spores. One recent example involves assemblies of gold nanoparticles on a gold substrate for the highly sensitive detection of dipicolinic acid (DPA), a biomarker for bacterial spores such as Bacillus anthracis. This type of substrate exploits a strong SERS effect produced by the particle–particle and particle–substrate plasmonic coupling. It is capable of accurate speciation of the biomarker but also selective detection under various reactive or non-reactive conditions. In the case of detecting Bacillus subtilis spores, the limit of detection is quite comparable (0.1 ppb for DPA, and 1.5 × 109 spores per L (or 2.5 × 10−14 M)) with those obtained using silver nanoparticle-based substrates. Implications of the recent findings for improving the gold nanoparticle-based SERS substrates with ultrahigh sensitivity for the detection of bacterial spores are also discussed.

Graphical abstract: Nanoparticle-based substrates for surface-enhanced Raman scattering detection of bacterial spores

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Article information


Submitted
04 Apr 2012
Accepted
11 Jun 2012
First published
29 Jun 2012

Analyst, 2012,137, 3601-3608
Article type
Minireview

Nanoparticle-based substrates for surface-enhanced Raman scattering detection of bacterial spores

H. Cheng, S. Huan and R. Yu, Analyst, 2012, 137, 3601
DOI: 10.1039/C2AN35448A

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