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Issue 17, 2012
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Homogeneous assay for evaluation of aptamer–protein interaction

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Abstract

We introduce Amplified Luminescent Proximity Homogenous Assay (ALPHA) to assess the KD value of aptamer–protein complexes as demonstrated through the study of apple stem pitting virus coat protein-specific aptamers. This method can be used as a simple, cost-effective method for screening aptamer–target protein interactions during aptamer selection.

Graphical abstract: Homogeneous assay for evaluation of aptamer–protein interaction

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Publication details

The article was received on 28 Mar 2012, accepted on 04 Jul 2012 and first published on 06 Jul 2012


Article type: Communication
DOI: 10.1039/C2AN35419E
Analyst, 2012,137, 3929-3931

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    Homogeneous assay for evaluation of aptamer–protein interaction

    G. Lautner, Z. Balogh, A. Gyurkovics, R. E. Gyurcsányi and T. Mészáros, Analyst, 2012, 137, 3929
    DOI: 10.1039/C2AN35419E

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