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Issue 18, 2012
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Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell

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Abstract

Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) with the use of a slide-on germanium accessory followed by chemometric analysis allowed for providing meaningful information about the biochemical composition of a single endothelial cell. In this work, the methodology of the ATR-FTIR measurements of dried cells and dried cells immersed in water solution is presented. The contact of the cell and Ge crystal was set up manually and monitored through the integration of the amide I band. Additionally, the cell imaging in transreflection mode was tested, but the spectral differences between sub-cellular structures were not prominent in the registered spectra. It has been shown that the ATR-FTIR method gives better results due to the increased spatial resolution and S/R ratio as well as small contribution of the optical artifacts.

Graphical abstract: Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell

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Publication details

The article was received on 03 Dec 2011, accepted on 27 Jun 2012 and first published on 28 Jun 2012


Article type: Communication
DOI: 10.1039/C2AN35331H
Analyst, 2012,137, 4135-4139

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    Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell

    T. P. Wrobel, K. M. Marzec, K. Majzner, K. Kochan, M. Bartus, S. Chlopicki and M. Baranska, Analyst, 2012, 137, 4135
    DOI: 10.1039/C2AN35331H

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