Issue 13, 2012

Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary electrophoresis

Abstract

We present a design for a sheath-flow cuvette that uses a relatively inexpensive quartz cuvette. The cuvette has a high optical quality square flow chamber that is fused to quartz tubes at each end. PEEK/TEFZEL fittings hold and seal the quartz flow chamber without putting strain on the cuvette. The performance of the cuvette is evaluated as a laser-induced fluorescence detector for capillary electrophoresis. The cuvette produces mass detection limits of 50 yoctomoles (30 copies) for 5-carboxyl tetramethylrhodamine (5 TAMRA SE) with a separation efficiency of 400 000 theoretical plates.

Graphical abstract: Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary electrophoresis

Article information

Article type
Paper
Submitted
06 Mar 2012
Accepted
11 Apr 2012
First published
13 Apr 2012

Analyst, 2012,137, 3099-3101

Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary electrophoresis

O. O. Dada, B. J. Huge and N. J. Dovichi, Analyst, 2012, 137, 3099 DOI: 10.1039/C2AN35321K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements