Issue 12, 2012
From the journal:

Analyst

Sequential elution of multiply and singly phosphorylated peptides with polar-copolymerized mixed-mode RP18/SCX material†

Xiuling Li,*a   Zhimou Guo,a   Qianying Sheng,b   Xingya Xuea  and  Xinmiao Liang*ab  

* Corresponding authors

a Key Lab of separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, 457 Zhongshan Road, Dalian 116023, China
E-mail: liangxm@dicp.ac.cn

b Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

Abstract

Novel polar-copolymerized mixed-mode RP18/SCX material was developed for feasible phosphopeptide enrichment, in which multiply and singly phosphorylated peptides could be sequentially eluted and separated with high selectivity.

Graphical abstract: Sequential elution of multiply and singly phosphorylated peptides with polar-copolymerized mixed-mode RP18/SCX material

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Article information

DOI
https://doi.org/10.1039/C2AN35247H
Article type
Communication
Submitted
21 Feb 2012
Accepted
10 Apr 2012
First published
11 Apr 2012

Analyst, 2012,137, 2774-2776

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Sequential elution of multiply and singly phosphorylated peptides with polar-copolymerized mixed-mode RP18/SCX material

X. Li, Z. Guo, Q. Sheng, X. Xue and X. Liang, Analyst, 2012, 137, 2774 DOI: 10.1039/C2AN35247H

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