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Issue 15, 2012
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Surface functionalization chemistries on highly sensitive silica-based sensor chips

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Abstract

The surfaces of silica-based sensor chips, designed for evanescent-field-coupled waveguide-mode sensors, were functionalized using various surface chemistries. The immobilization of molecular entities on the functionalized silica surfaces was monitored using various microscopic techniques (scanning electron, fluorescence, and atomic force microscopies). Further, gold nanoparticle-based signal enhancement analyses were performed with protein conjugation on different functionalized surfaces using a waveguide-mode sensor. Based on these analyses, the sensor surfaces modified with glutaraldehyde (Glu) and carbonyldiimidazole were found to be good for molecules of different sizes. In addition, it can be inferred that the Glu-modified surface may be suitable for small molecules with diameters around 5 nm owing to its surface roughness. The modified surface with carbonyldiimidazole is suitable for the direct immobilization of larger molecules especially for biomolecular assemblies without intermediate chemical modifications.

Graphical abstract: Surface functionalization chemistries on highly sensitive silica-based sensor chips

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Publication details

The article was received on 05 Dec 2011, accepted on 06 May 2012 and first published on 08 May 2012


Article type: Paper
DOI: 10.1039/C2AN35159E
Citation: Analyst, 2012,137, 3520-3527

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    Surface functionalization chemistries on highly sensitive silica-based sensor chips

    S. C. B. Gopinath, K. Awazu, M. Fujimaki, K. Shimizu, W. Mizutani and K. Tsukagoshi, Analyst, 2012, 137, 3520
    DOI: 10.1039/C2AN35159E

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