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Issue 21, 2012
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Introducing cymantrene labels into scattering scanning near-field infrared microscopy

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Abstract

In this paper we investigate metal–organic compounds as infrared (IR) active labels by scattering scanning near-field infrared microscopy (IR s-SNOM, often also abbreviated as s-SNIM) with a lateral resolution of 90 × 90 nm2. Tailor-made IR spectroscopic probes based on cymantrene (CpMn(CO)3 with Cp = η5-C5H5) conjugated to a cysteine-modified pseudoneurotensin (pNT-Cys-OH) peptide were prepared by automated microwave-assisted solid phase peptide synthesis (SPPS) and characterized by HPLC, ESI-MS and IR. Well-defined patterned self-assembled monolayers on a gold surface were prepared by microcontact printing of 1-octadecanethiol (ODT) followed by additional incubation in ethanolic solution of the cymantrene–peptide derivative. The self-assembled monolayers have been evidenced by infrared reflection absorption spectroscopy (IRRAS) and AFM. CO laser source radiation was tuned (1944, 1900, 1798 and 1658 cm−1) for imaging contrast with good matching correlation between spectroscopic and topographic patterns at specific characteristic metal carbonyl and amide bands (1944 cm−1 (λ = 5.14 μm) and 1658 cm−1 (λ = 6.03 μm)). Cymantrene probes provide an attractive method to tag a unique spectroscopic feature on any bio(macro)molecule. Introducing such probes into super-resolution IR s-SNOM will enable molecular tracking and distribution studies even in complex biological systems.

Graphical abstract: Introducing cymantrene labels into scattering scanning near-field infrared microscopy

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Article information


Submitted
05 Dec 2011
Accepted
23 Aug 2012
First published
11 Sep 2012

Analyst, 2012,137, 4995-5001
Article type
Paper

Introducing cymantrene labels into scattering scanning near-field infrared microscopy

I. Kopf, H. W. Peindy N'Dongo, F. Ballout, U. Schatzschneider, E. Bründermann and M. Havenith, Analyst, 2012, 137, 4995
DOI: 10.1039/C2AN16201F

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