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Issue 4, 2012
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Autoantibody detection by direct counting of antigen-displayed yeast cells

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Abstract

In this study, we report a new immunoassay platform based on yeast surface display technology for detection of autoantibodies involved in autoimmune diseases, e.g., systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS). The autoantigens of Ro52/SSA epitope and SmD were chosen to be displayed on the yeast surface with their respective antibodies as the analytes. By using magnetic beads modified with protein G, yeast cells bound with specific target antibody can be captured. The amount of analytes could be determined by counting the number of fluorescent yeast cells captured in a magnetic field. The platform showed promising results in the detection of SLE autoantibodies with high sensitivity and multiplex detection capability over the traditional approaches.

Graphical abstract: Autoantibody detection by direct counting of antigen-displayed yeast cells

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Publication details

The article was received on 10 Oct 2011, accepted on 22 Nov 2011 and first published on 22 Dec 2011


Article type: Paper
DOI: 10.1039/C2AN15946E
Analyst, 2012,137, 999-1004

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    Autoantibody detection by direct counting of antigen-displayed yeast cells

    D. Cheng, Y. Guo and I. Hsing, Analyst, 2012, 137, 999
    DOI: 10.1039/C2AN15946E

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