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Issue 4, 2012
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Autoantibody detection by direct counting of antigen-displayed yeast cells

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Abstract

In this study, we report a new immunoassay platform based on yeast surface display technology for detection of autoantibodies involved in autoimmune diseases, e.g., systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS). The autoantigens of Ro52/SSA epitope and SmD were chosen to be displayed on the yeast surface with their respective antibodies as the analytes. By using magnetic beads modified with protein G, yeast cells bound with specific target antibody can be captured. The amount of analytes could be determined by counting the number of fluorescent yeast cells captured in a magnetic field. The platform showed promising results in the detection of SLE autoantibodies with high sensitivity and multiplex detection capability over the traditional approaches.

Graphical abstract: Autoantibody detection by direct counting of antigen-displayed yeast cells

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Article information


Submitted
10 Oct 2011
Accepted
22 Nov 2011
First published
22 Dec 2011

Analyst, 2012,137, 999-1004
Article type
Paper

Autoantibody detection by direct counting of antigen-displayed yeast cells

D. Cheng, Y. Guo and I. Hsing, Analyst, 2012, 137, 999 DOI: 10.1039/C2AN15946E

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