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Issue 3, 2012
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Evolution of quantitative methods in protein secondary structure determination via deep-ultraviolet resonance Raman spectroscopy

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Abstract

Deep-ultraviolet resonance Raman (DUVRR) spectra is sensitive to secondary structural motifs but, similar to circular dichroism (CD) and infrared spectroscopy, requires the application of multivariate and advanced statistical analysis methods to resolve the pure secondary structure Raman spectra (PSSRS) for determination of secondary structure composition. Secondary structure motifs are selectively enhanced by different excitation wavelengths, a characteristic that inspired the first methods for quantifying secondary structures by DUVRR. This review traces the evolution of multivariate methods and their application to secondary structure composition analyses of proteins by DUVRR spectroscopy from the first experiments using two-wavelengths, and culminating with recent studies utilizing time-resolved DUVRR measurements.

Graphical abstract: Evolution of quantitative methods in protein secondary structure determination via deep-ultraviolet resonance Raman spectroscopy

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Publication details

The article was received on 19 Aug 2011, accepted on 18 Nov 2011 and first published on 06 Dec 2011


Article type: Critical Review
DOI: 10.1039/C1AN15755H
Citation: Analyst, 2012,137, 555-562

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    Evolution of quantitative methods in protein secondary structure determination via deep-ultraviolet resonance Raman spectroscopy

    C. A. Roach, J. V. Simpson and R. D. JiJi, Analyst, 2012, 137, 555
    DOI: 10.1039/C1AN15755H

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