Issue 22, 2011

Development of a highly selective cell-permeable ratiometric fluorescent chemosensor for oxorhenium(v) ion

Abstract

A novel 6-(2-pyridinyl)-5,6-dihydrobenzimidazo[1,2-c]quinazoline (HL) serves as a first-time highly selective and sensitive ratiometric fluorescent chemosensor probe for oxorhenium (ReO(V)) ion in acetonitrile : water = 9 : 1 (v/v) at 25 °C. The decrease in fluorescence at 410 nm and increase in fluorescence at 478 nm with an isoemissive point at 444 nm in the presence of ReO(V) ion is accounted for by the formation of mononuclear [ReOL2Cl] complex, characterized by physico-chemical and spectroscopic tools. The fluorescence quantum yield of the chemosensor (HL) was only 0.198 at 410 nm, and it increased more than 3-fold in the presence of 2 equiv. of the ReO(V) ion at 478 nm. Interestingly, the introduction of other metal ions and relevant anions caused the fluorescence intensity at 478 nm to be either unchanged or weakened. The fluorescence-response fits a Hill coefficient of 2.088 indicates the formation of a 1 : 2 stoichiometry for the L-ReO(V) complex. In the concentration range of 0–20 μM of oxorhenium(V) species calibration graph was linear with correlation coefficient (R) of 0.99994 and the calibration sensitivity was found to be 4.0 × 10−7 M. The cellular image in the confocal microscope clearly indicated the presence of ReO(V) in Candida albicanscells using this chemosensor (HL).

Graphical abstract: Development of a highly selective cell-permeable ratiometric fluorescent chemosensor for oxorhenium(v) ion

Supplementary files

Article information

Article type
Paper
Submitted
16 Jul 2011
Accepted
16 Aug 2011
First published
04 Oct 2011

Analyst, 2011,136, 4839-4845

Development of a highly selective cell-permeable ratiometric fluorescent chemosensor for oxorhenium(V) ion

S. Sen, T. Mukherjee, S. Sarkar, S. K. Mukhopadhyay and P. Chattopadhyay, Analyst, 2011, 136, 4839 DOI: 10.1039/C1AN15609H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements