Issue 15, 2011

A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles

Abstract

Gold nanoparticles (Au-NPs) with negative charges aggregate in the presence of Arg6 due to electrostatic interactions resulting in the red-shift of the plasmon absorption. But, after incubation of Arg6 with trypsin the aggregation of Au-NPs can be prohibited. Accordingly, a newly designed-Au-NPs based colorimetric assay method for trypsin activity is established. Trypsin with a concentration as low as 1.6 ng mL−1 can be assayed with this new colorimetric assay. This colorimetric label-free assay for trypsin can be performed in aqueous solution and both Au-NPs and Arg6 are easily accessible. Thus, this assay method is useful for screening inhibitors of trypsin.

Graphical abstract: A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
20 Mar 2011
Accepted
20 May 2011
First published
22 Jun 2011

Analyst, 2011,136, 3136-3141

A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles

W. Xue, G. Zhang and D. Zhang, Analyst, 2011, 136, 3136 DOI: 10.1039/C1AN15224F

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