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Issue 11, 2011
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Y-type, C-rich DNA probe for electrochemical detection of silver ion and cysteine

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Abstract

An electrochemical assay for the detection of silver ion was reported, which was based on the interaction of the Y-type, C-rich ds-DNA with Ag+. Upon addition of Ag+, Y-type, C-rich ds-DNA could form an intramolecular duplex, in which Ag+ can selectively bind to cytosinecytosine (C–C) mismatches forming C–Ag+–C complex. The binding result was evaluated by electrochemical impedance spectroscopy (EIS) and analyzed with the help of Randles' equivalent circuits. The differences of charge transfer resistance, ΔRCT, after and before the addition of Ag+, allows the detection and quantitative analysis of Ag+ with a detection limit of 10 fM. Moreover, cysteine (Cys) was applied to remove Ag+ from the C–Ag+–C complex, which allowed the Ag+ sensor to be reproduced. In the same way, ΔRCT for the C–Ag+–C system in the absence and presence of Cys allows the detection of Cys at a concentration as low as 100 fM. Finally, the potential application of the Ag+ sensor was also explored, such as in lake and drinking water.

Graphical abstract: Y-type, C-rich DNA probe for electrochemical detection of silver ion and cysteine

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Supplementary files

Article information


Submitted
23 Feb 2011
Accepted
01 Apr 2011
First published
21 Apr 2011

Analyst, 2011,136, 2242-2246
Article type
Paper

Y-type, C-rich DNA probe for electrochemical detection of silver ion and cysteine

H. Gong and X. Li, Analyst, 2011, 136, 2242
DOI: 10.1039/C1AN15159B

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