Issue 12, 2011
From the journal:

Analyst

Detecting DNA methylation through changes in transverse proton relaxation†

Hamsa Jaganathan,a   Pengfei Wang,a   Jonathon Klein,b   Amy C. Lossieb  and  Albena Ivanisevic*ac  

* Corresponding authors

a Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA

b Department of Animal Sciences, Purdue University, West Lafayette, IN, USA

c Department of Chemistry, Purdue University, West Lafayette, IN, USA
E-mail: albena@purdue.edu

Abstract

We present a facile, simple method to detect DNA methylation by measuring the transverse proton relaxation behaviour. Positively charged nanoparticles are arranged along the negatively charged backbone of DNA strands through electrostatic interactions. The arrangement of NPs along DNA strands aids to amplify and compare the transverse proton relaxation signal for un-cut versus cut DNA strands cleaved by sequence specific restriction enzymes. Results from this study suggest that the presence of methylation on DNA can be detected using superparamagnetic NPs using NMR.

Graphical abstract: Detecting DNA methylation through changes in transverse proton relaxation

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Article information

DOI
https://doi.org/10.1039/C1AN15146K
Article type
Communication
Submitted
18 Feb 2011
Accepted
18 Apr 2011
First published
03 May 2011

Analyst, 2011,136, 2441-2445

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Detecting DNA methylation through changes in transverse proton relaxation

H. Jaganathan, P. Wang, J. Klein, A. C. Lossie and A. Ivanisevic, Analyst, 2011, 136, 2441 DOI: 10.1039/C1AN15146K

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