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Issue 7, 2011
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Cetirizine as pH-dependent cross-reactant in a carbamazepine-specific immunoassay

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Abstract

High performance liquid chromatography (HPLC) was hyphenated with a previously reported carbamazepine-specific enzyme-linked immunosorbent assay (ELISA) as a screening approach to water analysis in order to identify possible interferences from transformation products. Treated wastewater was analysed and three substances were recognized by the antibody besides carbamazepine: the metabolites 10,11-dihydro-10,11-epoxycarbamazepine and 2-hydroxycarbamazepine plus the structurally not obviously related antihistamine cetirizine. The molar cross-reactivity against cetirizine was found to be pH-dependent and assessed to be 400% at pH 4.5 and 22% at pH 10.5. Performing the ELISA at pH 10.5 greatly improved the accuracy when carbamazepine was determined in surface and wastewater samples.

Graphical abstract: Cetirizine as pH-dependent cross-reactant in a carbamazepine-specific immunoassay

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Publication details

The article was received on 19 Nov 2010, accepted on 26 Jan 2011 and first published on 17 Feb 2011


Article type: Paper
DOI: 10.1039/C0AN00928H
Citation: Analyst, 2011,136, 1357-1364

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    Cetirizine as pH-dependent cross-reactant in a carbamazepine-specific immunoassay

    A. Bahlmann, J. Falkenhagen, M. G. Weller, U. Panne and R. J. Schneider, Analyst, 2011, 136, 1357
    DOI: 10.1039/C0AN00928H

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