Issue 7, 2011

A novel cross-H-channel interface for flow injection-capillary electrophoresis to reduce sample requirement and improve sensitivity

Abstract

In this study, a cross-H-channel interface was constructed for coupling flow injection with capillary electrophoresis (FI–CE) to reduce sample requirement and sensitivity loss in the typical FI–CE. Based on this cross-H-channel interface, a new FI–CE system was established, in which sample introduction was performed by directly injecting sample solution along a thin capillary (50 μm, i.d.) to the interface from an injection syringe. The sample requirement was reduced distinctly and usual sample dilution in the sample transport process was obviously decreased, thereby spontaneously enhancing the sensitivity. Moreover, because of the unique construction of the cross-H-channel interface, field amplified sample stacking (FASS) and high-speed CE were skillfully combined to further improve the sensitivity and to shorten separation time. The versatility of this new FI–CE was demonstrated by determination of ephedrine (E) and pseudoephedrine (PE) in human urine. Up to 45 repeated injections per hour and clearly baseline separation of E and PE in less than 1 min were achieved, giving limits of detection (LODs) of 0.23 and 0.21 μg mL−1 for E and PE, respectively, and yielding relative standard deviation (RSD) values of the migration time and the peak height (n = 5) of 2.6% and 3.1% for E, 2.3% and 3.3% for PE, respectively. In contrast to typical FI–CE, approximately 8–250-fold decreases in sample volume requirement, 7-fold shortening in separation time and 50-fold improvements in sensitivity were obtained.

Graphical abstract: A novel cross-H-channel interface for flow injection-capillary electrophoresis to reduce sample requirement and improve sensitivity

Article information

Article type
Paper
Submitted
01 Aug 2010
Accepted
13 Jan 2011
First published
08 Feb 2011

Analyst, 2011,136, 1322-1328

A novel cross-H-channel interface for flow injection-capillary electrophoresis to reduce sample requirement and improve sensitivity

H. Zhu, W. Lü, H. Li, Y. Ma, S. Hu, H. Chen and X. Chen, Analyst, 2011, 136, 1322 DOI: 10.1039/C0AN00592D

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