Issue 4, 2011

The potential of magnetic nanocluster and dual-functional protein-based strategy for noninvasive detection of HBV surface antibodies

Abstract

Magnetic nanoclusters (MNCs) were synthesized in a one-pot process, carboxylic MNCs and dual-functional protein were prepared and used to capture hepatitis B virus surface antibodies (anti-HBs) in simulated diseased oral mucosal transudate (OMT) samples. The specific substrate of dual-functional protein, dual-labeled double-chained DNA molecules, based on Fluorescence Resonance Energy Transfer (FRET), was used to amplify the detection signal and the detection limit of 0.1 ng mL−1 of anti-HBs monoclonal antibodies was achieved. Combination MNCs with dual-functional protein enables the noninvasive detection of hepatitis B virus (HBV) surface antibodies in OMT samples, showing promise as a diagnostic tool for the OMT diagnosis of infectious diseases with sensitive, specific and facile capabilities.

Graphical abstract: The potential of magnetic nanocluster and dual-functional protein-based strategy for noninvasive detection of HBV surface antibodies

Supplementary files

Article information

Article type
Communication
Submitted
15 Jul 2010
Accepted
20 Oct 2010
First published
03 Nov 2010

Analyst, 2011,136, 679-683

The potential of magnetic nanocluster and dual-functional protein-based strategy for noninvasive detection of HBV surface antibodies

H. Hu, H. Yang, D. Li, K. Wang, J. Ruan, X. Zhang, J. Chen, C. Bao, J. Ji, D. Shi and D. Cui, Analyst, 2011, 136, 679 DOI: 10.1039/C0AN00517G

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