Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 6, 2011
Previous Article Next Article

Highly sensitive routine method for urinary 3-hydroxybenzo[a]pyrene quantitation using liquid chromatography-fluorescence detection and automated off-line solid phase extraction

Author affiliations

Abstract

Many workers and also the general population are exposed to polycyclic aromatic hydrocarbons (PAHs), and benzo[a]pyrene (BaP) was recently classified as carcinogenic for humans (group 1) by the International Agency for Research on Cancer. Biomonitoring of PAHs exposure is usually performed by urinary 1-hydroxypyrene (1-OHP) analysis. 1-OHP is a metabolite of pyrene, a non-carcinogenic PAH. In this work, we developed a very simple but highly sensitive analytical method of quantifying one urinary metabolite of BaP, 3-hydroxybenzo[a]pyrene (3-OHBaP), to evaluate carcinogenic PAHs exposure. After hydrolysis of 10 mL urine for two hours and concentration by automated off-line solid phase extraction, the sample was injected in a column-switching high-performance liquid chromatography fluorescence detection system. The limit of quantification was 0.2 pmol L−1 (0.05 ng L−1) and the limit of detection was estimated at 0.07 pmol L−1 (0.02 ng L−1). Linearity was established for 3-OHBaP concentrations ranging from 0.4 to 74.5 pmol L−1 (0.1 to 20 ng L−1). Relative within-day standard deviation was less than 3% and relative between-day standard deviation was less than 4%. In non-occupationally exposed subjects, median concentrations for smokers compared with non-smokers were 3.5 times higher for 1-OHP (p < 0.001) and 2 times higher for 3-OHBaP (p < 0.05). The two urinary biomarkers were correlated in smokers (ρ = 0.636; p < 0.05; n = 10) but not in non-smokers (ρ = 0.09; p > 0.05; n = 21).

Graphical abstract: Highly sensitive routine method for urinary 3-hydroxybenzo[a]pyrene quantitation using liquid chromatography-fluorescence detection and automated off-line solid phase extraction

Back to tab navigation

Article information


Submitted
21 Jun 2010
Accepted
15 Dec 2010
First published
25 Jan 2011

Analyst, 2011,136, 1183-1191
Article type
Paper

Highly sensitive routine method for urinary 3-hydroxybenzo[a]pyrene quantitation using liquid chromatography-fluorescence detection and automated off-line solid phase extraction

D. Barbeau, A. Maître and M. Marques, Analyst, 2011, 136, 1183
DOI: 10.1039/C0AN00428F

Social activity

Search articles by author

Spotlight

Advertisements